Improve your protein production
Our unique PlugBug® platform is a true game-changer - enabling you to identify and scale-up biopharmaceuticals and industrial enzymes like never before.
Sometimes identifying the strongest microorganisms can feel like searching for a needle in a haystack.
Traditionally it takes a great deal of time, effort (and some luck) to find the best strains with the best protein expression, optimum development timescales and good reliability.
At DSM we’ve developed a game-changing protein platform that addresses and solves these issues for our customers: PlugBug®: As the name suggests, it works by simply and quickly “plugging” the recombinant DNA encoding for your protein into our “bugs”.
The result is an elite family of fungi and bacteria known as our PlugBugs® - all based on standardized, high cell-density fermentation protocols.
True process development
PlugBug® is more than just a research platform. It also enables true process development, with scalability to production levels in mind.
To this end we’ve developed standardized, high cell-density fermentation protocols, with proven performance at production scale, with no intermediate step required - saving you time and money.
mammalian
|
microbial
|
up to scale |
|
| Escherichia coli | 3 - 10 g/l | 5 - 20 g/l | 25 m3 |
| Bacillus subtilis | 0.2 - 4 g/l | 2 - 20 g/l | 100 m3 |
| Pichia pastoris | 0.2 - 5 g/l | 1 - 15 g/l | 25 m3 |
| Kluyveromyces lactis | 0.5 - 3 g/l | 3 - 15 g/l | 100 m3 |
| Aspergillus niger | 0.3 - 4 g/l | 5 - 50 g/l | 100 m3 |
Other species
Aside from our PlugBugs®, there are various other microorganisms that we’ve used successfully as host cells for proteins production:
Bacteria: various species of Bacillus, Lactobacillus, and Lactococcus
Fungi: Saccharomyces cerevisiae, Yarrowia lipolytica, Trichoderma reesei
