Biotin is a sulfur-containing imidazole derivative. Biotin, with its unique structure, contains three asymmetric carbon atoms; therefore, eight isomers are possible (Illus. 1). Of these isomers only one, d-biotin, which has no specific unit of activity, has vitamin acitivity. Thus, 1 g of d-biotin equals 1 g of activity. The stereoisomer l-biotin is inactive.