Vitamin D2 did not promote growth as well as an equal amount of D3 when fed to catfish (Andrews et al., 1980). Brown and Robinson (1992) did not find a difference between the ability of D2 and D3 to promote growth in catfish. Based on growth, D3 was 3.27 times as potent as D2 in rainbow trout (Barnett et al., 1982a).
Certainly it appears that water chemistry in particular dramatically affects vitamin D metabolism in the fish and its interaction with calcium uptake and regulation.