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To get a quick answer to your question, take a look at these frequently asked questions.
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| 1 | How to get easier meat juice with the meat press? |
| 2 | Can I use water for the negative control? |
| 3 | What to do with a doubtful result? |
| 4 | Can I detect X antibiotic with the test? |
| 5 | Can I freeze my sample and test later? |
| 6 | Can I use another incubator instead of the Premi®Test-incubator delivered with the Starterkit Premi®Test? |
| 7 | Can I incubate the test at 60°C? |
| 8 | Can I skip using the foil during the incubation? |
| 9 | Can I store the Premi®Test in my laboratory at room temperature? |
| 10 | Is the Premi®Test system robust? |
| 11 | Do cleaning agents and disinfectants affect the Premi®Test? |
| 12 | How do I have to pipette the meat juice on the ampoule? |
| 13 | How can I determine more specific which antibiotic is in the ampoule after having positive results? |
| 14 | Can I test other types of samples? |
| 15 | Can I test eggs white / egg yolk or do I have to test the whole egg? |
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| 1 | How to get easier meat juice with the meat press? |
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Preheat the press before usage and keep the press upside-down during pressing.
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| 2 | Can I use water for the negative control? |
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No you cannot. We recommend to use meat-juice earlier tested negative with the Premi®Test as a standard.
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| 3 | What to do with a doubtful result? |
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A doubtful result indicates the presence of anti microbial compounds in low concentrations or some inhibiting effect of the matrix. Incubate the test 5 minutes longer for a clearer result or retest the sample.
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| 4 | Can I detect X antibiotic with the test? |
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The product sheet gives a broad overview of antibiotics, which can be detected. If wanted antibiotic is not present in this table you can look for the detection limit of antibiotics present in the same antibiotic group. This can give an indication for the detection limit. In most cases the detection limit of Premi®Test for antibiotic within the same antibiotic group are in the same ranges.
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| 5 | Can I freeze my sample and test later? |
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Yes you can, if the sample is packaged correctly. Be careful with thawing, avoid condensation water on your sample.
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| 6 | Can I use another incubator instead of the Premi®Test-incubator delivered with the Starterkit Premi®Test? |
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No, It is better to use the delivered Premi®Test-incubator or a water bad at 64 °C since the temperature is more homogeneous and better to control.
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| 7 | Can I incubate the test at 60°C? |
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No, the incubation temperature has to be at 64°C +/- 0.5°C.
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| 8 | Can I skip using the foil during the incubation? |
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No you cannot, if the agar will become to dry you will not get the right results.
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| 9 | Can I store the Premi®Test in my laboratory at room temperature? |
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No you cannot; the test has to be stored in the refrigerator preferably between
4 - 8 °C. Otherwise the shelf live of the test can decrease.
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| 10 | Is the Premi®Test system robust? |
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Yes it is, but avoid extreme rough handling with the Premi®Test. When the test agar gets loose from the ampoule wall, the Premi®Test will not work properly any more.
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| 11 | Do cleaning agents and disinfectants affect the Premi®Test? |
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Yes, any anti microbial compounds can inhibit the Premi®Test and can lead to false positive results.
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| 12 | How do I have to pipette the meat juice on the ampoule? |
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Empty the syringe carefully against the wall of the ampoule. Do not touch the agar.
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| 13 | How can I determine more specific which antibiotic is in the ampoule after having positive results? |
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To confirm the presence of beta-lactam antibiotics:
Mix one ml of the sample with 0.1 ml of a solution of 50 International Units of penicillinase type 1. (ex Bacillus cereus) per ml water. Test 0.1 ml of the mixture with the Premi®Test. If this test gives a yellow color, it confirmed the presence of beta-lactam antibiotic (penicillin, ampicillin, cloxacillin, cephapirin, cefuroxine etc.) in the sample.
To confirm the presence of sulphonamides:
Make an aqueous solution containing 1 mg of the sodium salt of p-amino benzoic acid (PABA). Test 0,1 ml of a mixture of 1 ml sample and 0,1 ml of the PABA solution. If this test gives a yellow color, it confirmed the presence of sulphonamide, a sulphone or an antifolate in the sample.
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| 14 | Can I test other types of samples? |
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Yes, you can use the Premi®Test for urine, blood, feed and honey (click here for the brochures and technical bulletins for pre-treatments). These can be tested after neutralization of disturbing factors, such as acid or colour. In some cases it is necessary to dilute the sample and adjust the pH. It is recommended to include a negative control with the same type of sample, known to be inhibitor free, to determine the required incubation time.
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| 15 | Can I test eggs white / egg yolk or do I have to test the whole egg? |
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Since the EU MRL's are given for the whole egg, the egg white and egg yolk has to be tested together. The main issue is the homo-generousness of the sample (click here for the brochures and technical bulletins for pre-treatments).
It is possible to test the egg white and egg yolk separate. In this case you have to be aware that the pH of the egg white is much higher (around 9) than the pH of egg yolk (around 6). The Premi®Test is based on an acid / base indicator. When you test the egg white (pH 9) the incubation time can be increased (incubation time over 3 hours). So it is important that when you execute the test you take a negative control to determine the exact incubation time.
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