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How to use Delvotest

A sample to be tested with Delvotest can either be taken from an individual cow's milk or a tank. The subsequent steps to be taken are explained below. For your convenience, download the photo instruction.

milking from cow

Taking a sample of an individual cow's milk

When analysing cow's milk, the tank must contain samples from all four quarters as milk from non-treated quarters may also be infected. You should also be careful not to use the foremilk as this may give an inaccurate antibiotic reading.

milk from tank

Take a sample of tank milk

Stir the milk in the tank before taking a sample to ensure the sample taken is representative of the whole tank.

cutting ampoules

Step 1: Preparation
Cut off the required number of ampoules with a pair of scissors. Be careful not to damage the foil of the remaining ampoules.

making holes in seal

Step 2: Open the seal
Open ampoule(s) by punching a hole in the aluminum foil with the syringe. Mark the ampoules for sample identification.

milk sampling

Step 3: Take a milk sample
Attach a new disposable pipette to the syringe. Depress the plunger completely, dip the tip in the milk sample and allow the plunger to return slowly under pressure of the spring.

filling ampoules

Step 4: Inoculation
Empty the syringe into the correspondingly marked ampoule by slowly depressing the plunger of the syringe. Use a fresh disposable pipette for each milk sample.

run test

Step 5: Incubation
Check the temperature of the incubator (64ºC ±0.5ºC). Put the ampoule(s) into the incubator. Record the time and set timer for 3 hours or use control time.

test ready

Step 6: Results
After 3 hours, remove the samples from the incubator. Read the colour of the lower 2/3 part of the solid agar in the ampoule(s) after the required incubation time.


Results and interpretation

Interpreting the results

  • Fail: if the colour does not change (remains purple) the bacteria have not developed due to the presence of antibiotic residues.
  • Pass: if the colour changes (yellow) the bacteria have developed, indicating no antibiotic residues in the sample.

Colour interpretation
To make the colour interpretation easier, you can use the colour chart below. Click here to download a printable version of the chart.

colour chart Delvotest

Quick links

Photo instruction
(PDF: 78 Kb)


Order Delvotest